Human NOS1AP (Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein) ELISA Kit

Human NOS1AP (Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein) ELISA KitThe Human NOS1AP (Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein) ELISA Kit measures NOS1AP in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti NOS1AP antibody. Add standard and properly diluted sample into relevant well respectively. After

The Human NOS1AP (Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein) ELISA Kit measures NOS1AP in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti NOS1AP antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with NOS1AP bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of NOS1AP in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.