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Description
Human CD206 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Cluster of Differentiation 206 (CD206) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Cluster of Differentiation 206 (CD206) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Cluster of Differentiation 206 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | The CD206 molecule (CD206) is primarily found on the surface of macrophages, immature dendritic cells, and liver sinusoidal endothelial cells, but is also expressed on the surface of skin cells, such as dermal fibroblasts and keratinocytes. It is the first member of a family of endothelial cell receptors that includes Endo180 (CD280), M-type PLA2R, and DEC-205 (CD205). | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.1 ★★★★★
Based on 1547 reviews
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Product Reviews
★★★★★ 4
For those that really Want to know!
Format: Paperback
I chose this rating because of the excellence of content. This author has chosen to give us, those who are truly seeking answers to difficult questions, the possibilities in finding closure or agreement with the very difficult task of merging Science, and all it entails, with our faith. I always feel pulled both ways with ther being no logical way to blend the two, I then felt I must have to give up one for the other but could not do so. This book has helped me begin the journey of understanding what I've always known to be true but could not put together. They do work. There are logical explanations for the seeming opposites of scripture and science. It's a Very important read. For years I have wished C.S. Lewis was still alive. He i have turned to for so many things. But with so many advances since his death, I have needed new thoughts as like minded as he . There are more Lewises out there!!
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Reviewed in the United States on January 24, 2013
★★★★★ 3
Thought-provoking but misses its "target audience"
Format: Paperback
First, the good. This is a thought-provoking book that takes complex subject matter and makes it very easy to understand. In "The Evolution of Adam" Dr. Enns does an excellent job on many fronts - most notably giving a brief overview of the history of biblical criticism and its importance to the evolution debate. His ability to distill ideas down to the core was impressive. If I had to recommend to someone 50 pages on biblical criticism I might tell them to read the first portion of this book. However, as I read the book I kept wondering how the path he was taking would allow him to argue for an Evangelical perspective (as he says in the introduction). In short, he does not. Not even close.
Dr. Enns must not know his target audience very well if he thinks that this book is targeted for Evangelicals. Virtually none of the positions that he espouses in this book are even close to what an Evangelical Christian would be comfortable defending. He has little regard for any historicity behind any of the biblical accounts and frequently tosses out the phrase "most scholars agree" as a trump card. He does a good job of helping understand the culture and history that surrounded the biblical accounts yet in the end the reader is left wondering where story and history actually meet or if possibly the whole thing was simply conjured up for political reasons. In the end, I think the question the reader is left with is "does it matter if anything in the Bible ACTUALLY happened?". How you answer that may well determine how much you enjoy this book.
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Reviewed in the United States on January 24, 2012
★★★★★ 5
Peter Enns "Upends" Tradition!
Format: Paperback
One cannot but deeply admire what Peter Enns has managed to produce within the span of less than 150 pages - not counting his endnotes. Kudos as well for his penetrating exegetical insights...to say nothing as regards his courage: few conservative evangelicals (and even fewer fundamentalists) will find the title "The Evolution of Adam" something that warms the heart. And yet what Enns has produced here not only is revolutionary (in a very real sense - see below) but may well prove to be one of the more controversial books on the science/theology debate of recent years.
Why so? Primarily because (according to Enns - Part Two of his book) Paul's creative use (in Romans) of the Adam and Eve story in Genesis was primarily for apologetic purposes...a matter that will be discussed in greater detail below. But we begin with Part One.
Essentially Part One (four chapters) represents Enns' understanding of the crucial importance Ancient Near Eastern influences exerted upon the biblical writers - the writer/s of the Genesis creation account in particular. Enns (correctly in my view)hammers this point repeatedly for the reader to consider - i.e., the bible (the whole of it) was not written in a cultural vacuum unsullied by the surrounding culture/s of pagan religious thought, whether ancient Sumerian, Babylonian, or Greco-Roman. Indeed, to do otherwise would have been an impossibility - somewhat like trying to walk along the Tibetan foothills while refusing to breathe its polluted 'pagan' air. None of us ever fully escapes the surrounding influences of culture - and the bible was never intended to do so; rather, God (if one believes in biblical inspiration...as Enns does) works fully within the conceptual categories of culture. Hence, the two creation accounts in Genesis come to us fully embedded with the concepts of Ancient Near Eastern thought patterns. Perhaps the most we can say here is that the Genesis accounts represent (in varying ways) the "demythologizing" of prior Ancient Near Eastern accounts: the God of Israel is not to be identified with any aspect (sun, moon, stars, etc.) of the created order.
So far so good. There's nothing really new here that hasn't been said already by any number of conservative evangelical scholars. Part Two, however, is something entirely different. Here Enns focuses his attention on Paul's creative use of the Old Testament, seeing as how the death and resurrection of Christ has caused Paul to look at the OT writings from a radically different perspective - Romans 5:12-21 in particular.
These verses have a long, long history in the Christian Church as providing the church's understanding of how sin and death entered the world of human existence:
we all "inherited" sin and death in and through the disobedience of Adam back in Eden. Not so...says Enns. And here is where his account veers off in a direction entirely different from traditional orthodox belief - for, according to Enns, Paul gave a particular 'Pauline spin' to these verses that cannot be found either in the OT itself, or in the Second Temple Judaism of which Paul himself was a part. Because the death and resurrection of Christ radically altered Paul's understanding of God's redemptive work in the world he (Paul) "found" in the Adam story an ideal explanation for why it is all Jews and Gentiles alike share in the universal experience of sin and death. Therefore, Adam's disobedience in Eden is NOT the cause of the universal human experience of sin and death (per Enns); rather, the story of Adam's disobedience served Paul's apologetic purposes...quite apart from whatever the story's original intention might have been. The true "origin" of sin and death remains a mystery, for the answer is not to be found (indeed if it can be "found" at all!) in the early Genesis account of Adam and Eve.
And here is where we encounter the book's controversial nature, for Enns' view represents a dramatic departure from the traditional view - a traditional view that has a rich theological heritage that passes directly through the Reformation all the way back to Augustine.
As previously stated, I deeply admire and respect what Enns has done here. For the most part I think he is on the right track. Furthermore, he makes mention of the fact that recent developments in biology have strongly indicated that we cannot possibly trace all modern humans back to an original "Adam and Eve." However, we knew that already...quite apart from modern biology informing us of the fact. Anthropology and paleontology had already amassed considerable evidence that proto-humans and modern humans were spread across the earth long before any conceivable Adam and Eve could have existed. Apparently, however, modern biology speaks with a more powerful voice than anthropology; thus, we are seeing a spate of books recently on the topic of whether or not Adam and Eve were historical - Enns' book being only one of a growing number. (Due to the geneologies in early Genesis we are somewhat limited in "how far back" we can place an Adam and Eve. Placing them 25 to 40 thousand years into the past in order somehow to allow them to be the true ancestors of all modern humans does a grave injustice to the geneologies that plain and simply do not allow for this sort of radical time reversal - a matter that any number of evangelicals, who have done this sort of thing, seem unwilling to appreciate. The early Genesis geneologies, even allowing for some "gaps," serve as a control against such unwarranted time expansion. An Adam and Eve of perhaps 6 to 8 thousand BC appears to be about the limit of what we can reasonably expect). In any case, Enns has raised a thorny and difficult issue in a way previous books on the question have not, and I believe his book will contribute substantially to more open theological discussion (one hopes without heated rancor) on the debate. In the meanwhile, some final thoughts.
Personally, I find it more than a tad curious that David Rohl (a somewhat controversial Egyptologist) has recently authored a book (From Eden to Exile, Greenleaf Press) in which he strongly defends an historical Adam - and yet Rohl acknowledges that he is an atheist. All this is most strange: an evangelical scholar arguing against an historical Adam while an atheistic historian argues for one! ("What fools these mortals be!")
I happen to agree with much of what Enns writes. However, I think Rohl has a point- even though how he fleshes his historical Adam out is somewhat bizarre. For one thing, I'm not entirely comfortable (despite some of Enns' powerful arguments) with a geneology of Jesus in the Gospels that would include "fictious" characters who never even existed. (I might as well inform you that my great, great grandfather was Dr. Jekyll and my great, great, great grandfather was Mr. Hyde). I don't see why getting rid of an historical Adam is at all necessary. Enns himself offers the possibility that OT Israel viewed Adam as their senior partriarch - the man who originally started the "clan." I personally see great possibilities here via leaving Adam within historical existence as Israel's original, grand patriarch.
The origin of sin and death via the Adam and Eve story is another matter entirely. Biology and anthropology together appear to just plain and simply rule it out - and sticking Adam back into the age of the Cro-Magnons and Neaderthals in order to "save" the doctrine is a clear instance of an act of sheer desperation. But I see no reason why we necessarily have to conclude that the "origin" of sin and death (if that's the right word even to use...which I'm not even sure about) can only be regarded as lost in the misty past. I think there is a possible way forward here, and even via an historical Adam, while at the same time embracing what Enns is talking about. I think there may well be a way to retain a personal Adam (perhaps 6 to 8 thousand BC), while also showing how sin and death had their origin in him...but with an entirely different understanding that is informed by Enns' book. Unfortunately, spelling all that out is - like "The Evolution of Adam" - a book unto itself. And Amazon commentary is not the place where one is allowed to "write a book" - quite apart from how lengthy my own commentary here has been.
In the meanwhile...kudos again to Enns for his truly provocative and highly insightful contribution to the cause. His vigorous defense of the incarnation, the atonement, and the resurrection is profoundly gratifying. Because of his firm stance here no one can accuse him of being unorthodox!
(NOTE: Readers interested in a critical analysis of David Rohl's "From Eden to Exile: the 5000 Year History of the People of the Bible," and why this book is of such strategic importance for Old Testament studies - scholars in particular, can easily access my recent review of this book (titled "David Rohl: A "Maverick" in Search of History") by clicking on "See All My Reviews" directly above, or by going to the book's Amazon website.
Hope you enjoy the read!
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Reviewed in the United States on January 18, 2012
★★★★★ 5
A must-have for students and researchers
Format: Spiral-bound
I use this all the time. The Concise Guide to APA Style (7th Edition) is incredibly helpful, easy to navigate, and much less overwhelming than flipping through the full manual. Great quick reference for papers, citations, and formatting.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 16, 2026
★★★★★ 5
Perfect for learning APA format
Format: Spiral-bound
If you are one learning how to write, cite and use references in APA format this is the perfect book for you. It literally breaks down everything for you and has examples of what to do. It has an example essay if you need something to reference as well. I'd recommend this book to anyone that has a strict professor or that is learning how to write APA.
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Reviewed in the United States on February 15, 2026