Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 10 - Jul 15
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Rat 11βHSD1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (11βHSD1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (11βHSD1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat 11-Beta-Hydroxysteroid Dehydrogenase Type 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | 11β-Hydroxysteroid dehydrogenase type 1 (11βHSD1), also known as cortisone reductase, is an NADPH-dependent enzyme highly expressed in key metabolic tissues, including the liver, adipose tissue, and the central nervous system. In these tissues, it reduces cortisone to the active hormone cortisone, which activates the glucocorticoid receptor. It belongs to the family of short-chain dehydrogenases. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.2 ★★★★★
Based on 1084 reviews
Sort
Product Reviews
★★★★★ 5
Great Product
Size: 6 Pairs / 12 pcs
These banana plugs have worked perfectly, no matter what gauge I have run in them, they work great. Easy assembly and great quality.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 8, 2026
★★★★★ 4
The most legit banana plugs I could find on Amazon
Size: 6 Pairs / 12 pcs, Size: 6 Pairs / 12 pcs
NEW PREFACE:: I am kind of glad I first bought the horribly magnetic, horribly made and GOLD PAINTED Sewell crimp on plugs (some reviewers say that Sewell are better and easier to use than the FosPower, which is true in design, but in quality of materials FosPower beats them hands down!!) ... Changing from those to these FosPower plugs plus the Monoprice 90° plugs, I am flabbergasted at the difference they make in sound quality. I have heard people say that they can hear the difference and even as an audiophile I was skeptical..... but my skepticism has been completely erased by what I am hearing with my own ears. I waited a few days to make sure I wasn’t just tripping before updating this review 🤣....but it has become clear to me how much a difference plugs made from the right materials actually make!!! I am 100% serious that the bass coming out of my Polk Signature Series tower speakers has at least doubled at the same volume. I can also now turn the volume up Way past my max volume before, without any distortion and much more clarity and less harsh highs. Everything just sounds warmer in general now which is amazing!!!! This is dangerous info for me... because now I’m thinking about making some really nice custom wires to see how much of a difference that makes 🙄🙄🤣 OK onto the review of the actual plugs here~~
These seem like decent connectors. I can’t tell if they are brass or copper, but they only have a slight hint of a magnetic property when testing them with a very powerful magnet. I was easily able to fit x2 - 14ga wires into it for bi-wiring purposes, including screwing on the collar. (As seen in my example pic) The gold plating on the tips seems legit and not painted like some of the plugs from Sewell... I have tested and tried quite a few plugs from Amazon and most seem to be crap. These however hold up better than most! These and the Monoprice 90° banana plugs with the black and red rubber sleeves. The latter are gold plated copper and have zero magnetic properties. (if you need 90° banana plugs)
UPDATE: still the best plugs I have found, but upon full install found two issues. One is a defect where a set of the plug threads for the barrel cover body weren’t cut right. The body’s do not tighten and stay loose because of that. Obvious issue for vibration with metal on metal...
The other issue is that even though these say they can fit different gauge wires, they seem to be geared more towards 12 or 10 gauge. I am using semi solid strand 14 gauge and find that unless I fold the wire over to make it double thick, the bolts use up all the thread and seem to become kind of loose inside the body. They won’t fully seat and keep spinning no matter how much you tighten. They seem to stay secure still but I just wonder about longevity over time with vibrations. So I just doubled up the wire and they seated fine! Not the worst issue in the world, but just kind of a pain since you have to strip the wire longer than you normally would so you can fold it over.
ANOTHER UPDATE: I had to remove these plugs from the wire to connect them to a crappy spring terminal amp while my better amp was in the shop. The issue I wrote about with the set screws "bottoming out" on smaller gauge wire became a more pronounced issue! I could not unscrew the set screws anymore and just had to cut the wires... So that means I will need to purchase more of these to replace the ones I cannot get wire back into. Not the best design, but still one of the best plugs I have found on Amazon.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 26, 2021
★★★★★ 5
Sturdy. Tight fit. No wobble
Size: 2 Pairs / 4 pcs
These are the best ones I’ve found. This order will connect one wire. Luckily that’s what I needed. If you’re doing more than one wire…
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 18, 2026
★★★★★ 5
Superior Design!
Size: 6 Pairs / 12 pcs
Delivery was lightning fast.
I tried a pack of two pairs of these banana jacks as a sample to see if these would meet my needs. The design of these plugs is superior. The design was exactly what I wanted because the speaker wire is secured with TWO set screws. Then I ordered an additional six pairs of plugs to hook up my subwoofer to my amp and speakers.
(I feed the audio output from the amp to the sub. The sub has a crossover that keeps the low frequencies and sends the mid and treble frequencies to my bookshelf speakers.)
I love the security of two set screws on each plug and love the barrel that screws on AFTER the wire is secured by the set screws. Most banana plugs have a hole through the shaft of the plug that receives the stripped wire. Then the barrel cover is screwed down to secure the wire in the plug. This design compresses the speaker wire but eventually the cap loses it grip and allows the connection between the wire and the plug to become loose. Then the transfer of the audio signal becomes poor and the speakers sound bad. Every month or two I needed to check all the banana plugs and re-tighten many of them for the best performance. The two set screws eliminate this problem. The set screws are quite small and take a little extra care in installation. You will need a small flat blade screwdriver to tighten them. I used the 1/8" flat blade driver from a set of jewelers screwdrivers.
These well made plugs are a bit pricier that many of the banana plugs available but certainly not the priciest plugs available. For me, the slightly higher price was more than offset by the security of the wire within the plug.
I'm not thrilled with the extra tight fit of the tip on the plugs into the jacks. It took a lot of effort to insert the in the jack. Actually I "greased" the pins (very sparingly) with with non-corrosion, conductive paste (Ox-Gard, No-Corrode, etc. Do NOT use any other grease.) Next time I'll use pliers to compress the pin.
My only quibbles are that the spring pins that go into the jack are very tight and that the plugs are only sold in quantities of two pairs or 12 pairs.
I recommend these banana plugs for all who are using the banana plugs and jacks for hooking up your speakers. It's a very convenient way of connecting speakers if your speakers and amplifier have the banana jack as part of the binding post. Further, it's much safer to use banana plugs since the possibility of shorting your amplifier with a stray strand of wire is practically zero. Easy Five Stars.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 13, 2018
★★★★★ 5
Top banana
Size: 5 Pairs / 10 pcs
Theses banana plugs are hight quality non magnetic. Banana plugs easy to usexand very high quality sound different because of the better connection and quality.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 24, 2026
recommand products
Tibetan Spaniel - Bangle Bracelet
39.95
Neapolitan Mastiff - 18k Gold Finished Luxury Necklace
59.95
Brembo 11-13 BMW 135i Rear Premium UV Coated OE Equivalent Rotor
83.95
Brembo 12-16 Town & Country/12-20 Dodge Grand Caravan/12-14 Volkswagen Routan Rear UV Coated Rotor
76.96
Clumber Spaniel - 18k Gold Finished Luxury Necklace
59.95