Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 10 - Jul 15
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human Pancreatic Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) The extracted tissue must be placed in a pre cooled (2 8 C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it
Product Specification
| Usage |
1. Primary (1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm<(4) The tissue was digested with the digestion solution C of the primary tissue of the porcine endometrium, and shaken at 37 ℃ for 10-20 minutes (the digestion process was observed at any time)<(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion<(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge (7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board 2. Organ like passage culture (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4  & Nbsp& Nbsp& Nbsp& Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4 3. Organ like cryopreservation (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid (4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube (5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage 4. Organ like resuscitation (1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube<(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and 500 ul of porcine endometrial organoid culture medium A. |
||||||||||||||||
| Description |
Composition:
|
||||||||||||||||
| Storage Temp. | 4° C. 3months; -20° C. 1 year, see label for details |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.6 ★★★★★
Based on 1659 reviews
Sort
Product Reviews
★★★★★ 5
Just buy them
Color: Black,navy,burgundy,brown, Size: Small
Used to wear sliders all the time, figured i'd switch it up. These are super comfortable, probably the best material underwear I own. Great to just lounge around it or wear everyday. Fit is really good, I got a small and about 29-30" waist. Good bang for your buck too. I've washed them a handful of times and they hold up great, fit great and dont' shrink.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 7, 2026
★★★★★ 5
Good underwear. Husband likes them.
Color: Black, Size: Large
Husband wears XL and XL fit well. This is the second pack I got for him. They are decent quality, look and feel nice, not baggy yet not too tight anywhere. They are somewhat thin (but still supportive) and do not make him sweat more so he loves that. No problems washing (they didn’t shrink). Will continue to repurchase these.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 11, 2026
★★★★★ 5
Comfortable
Color: Black,navy,burgundy,brown, Size: Medium
I have had a lifetime of different briefs, some name brand, some generic.
I am 165 lbs, wear Medium, and these are closest to feeling commando of anything I’ve ever worn.
Comfortable, not too tight waist and legs, and yes silky touch yet not slinky.
Maybe not for support wear, but casual and cool comfort, the “Cat’s meow”.
I just came on to order some more
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 30, 2026
★★★★★ 3
Great fabric - pouch too small!
Color: Black, Size: X-Large
First, the fabric used is wonderfully soft and comfortable. It;s the first thing noticed when package was opened. Strange, but the elastic waistband is about 1/2", very similar to a woman's style panty. But it's comfortable and easily acceptable.
The main problem is that the pouch that holds your "stuff" in place, is oddly and unnaturally small. Small enough where this average man's "stuff" tends to fall out of the pouch on one side or the other. I sleep in my underwear, and every time I did with these, I awoke with the front pouch off to the side and everything down there was hanging outside of the underwear.
They also seem to do well through a wash cycle. However, if the main reason for wearing underwear is to keep your stuff in place, these fail from the beginning.
I ordered another brand from overseas, and they had the exact same problems as these! I wonder if that's just how they make them outside of the USA.
The Bottom Line?
Uncle Steve Disapproved!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 30, 2025
★★★★★ 5
Premium cotton underwear at a modest price. Highly recommend!
Color: Multicolora6-4 Pack, Size: X-Large
Bought my husband 2 variety packs of these, and they are still great over 2 years in. High quality cotton briefs are breathable, colors are vibrant, and the fit is spot on. A good value at this price point compared to designer brands, and the product overall exceeds expectations in terms of comfort level, durability and appearance.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 11, 2026
recommand products
Outlandos D'amour & Synchronicity
78.22
Wild Life (50Th Anniversary/Half-Speed Master) & Man On The Run (Ost) (180G)
74.18
Star Struck
102.50
A-Lex (2LP) & Quadra (40Th Anniversary Edition/Red Ruby LP Vinyl) & Machine Messiah (40Th Anniversary Edition/Red LP Vinyl)
116.62
Caravanserai (140G/Dl Code/Gatefold Sleeve) & Supernatural (2LP/150G) & Splendiferous (2LP/150G) & Blessings & Miracles
115.10